Legionella testing is the process of obtaining a water sample and analyzing it to detect the presence of the bacteria.
Legionella testing done to confirm effectiveness is defined as validation. Testing for Legionella is done to confirm that a Water Management Program is effective at controlling Legionella, which is defined as a hazard. It is also done to discover the source of exposure after a disease case is reported.
There are two general types of Legionella tests: Microbial culture tests (spread plates and field sampler “dipslides”) and molecular tests (Polymerase Chain Reaction, PCR). For microbial culture, L. pneumophila serogroup 1, serogroups 2-14 and Legionella spp. should always be determined for every isolate.
An INDEPENDENT, third-party testing service provides improved defensibility by removing real or perceived commercial conflict of interest or bias toward proprietary water treatment solutions.
Sampling locations are decided by the Water Management Team. Locations may include incoming water, showers, misters, ice machines, faucets, cooling towers and decorative water features. Phigenics recommends collecting post-flush samples from plumbing fixtures, when Legionella testing is done as part of routine validation to confirm WMP effectiveness. Call us to learn why.
Testing should be undertaken after a Water Management Team has confirmed that a program has been implemented as designed (verification). The frequency of testing is determined by the Water Management Team and is often monthly, quarterly or semi-annually.
Any response should be predetermined and documented as a “contingency response” in the Water Management Program. Contingency responses should include a review of the verification data and may include activities such as flushing or hyperchlorinating the water system. Call us to learn more.
The CDC has five considerations for laboratory expertise: ELITE accreditation, the performance of culture method, level of identification, willingness to save samples and experience with environmental risk assessment and sampling. Click here to see how Phigenics meets the CDC’s criteria.
PVT VIABLE (Viability Identification Assay By Legionella Enrichment) detects viable Legionella bacteria found in building water systems in as little as 72 hours. By combining traditional culture techniques with LegiPlex™, this new method results in our fastest turnaround time and highest sensitivity to date. This innovation also detects stressed Legionella bacteria that are unable to form colonies on the conventional ISO spread plate (Viable But Not Culturable; VBNC). False-negative results in the conventional culture method are now widely recognized as a serious problem because VBNC cells have been proven to be potentially infective and pathogenic.
PVT Next Day LegiPlex™ PCR delivers rapid results that confirm the serogroup of Legionella within building water systems. Polymerase Chain Reaction (PCR) analysis is a negative screening method that provides confirmation that there is no Legionella DNA in a water sample. High-Value PCR screening using PVT Next Day LegiPlex™ PCR is extremely cost-effective compared to standard culture testing and can be applied broadly across a building water system.
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Phigenics Analytical Services Laboratory (PASL) is certified as proficient in the Environmental Legionella Isolation Techniques Evaluation (ELITE) program administered by the CDC.
Phigenics performs the laboratory culture method for both its Phigenics patented PVT TimeZero™ field test and ISO 11731 spread plate method.
Phigenics performs comprehensive testing, including Legionella pneumophila (serogroups 1 and 2-14), non-pneumophila and Legionella species.
Phigenics saves all isolates of Legionella from all samples and has extensive experience in helping our clients work with public health laboratories and with the CDC.
Phigenics has extensive experience performing environmental risk assessments and sampling for Legionella with facilities of all different types and sizes.
Legionella Swab Test, Acinetobacter spp., Aspergillus spp., Burkholderia cepacia, Coliforms (total) & Escherichia coli, Fusarium spp., Heterotrophic Plate Count, Mycobacteria, Pseudomonas aeruginosa & Pseudomonas spp., Staphylococcus aureus, Stenotrophomonas maltophilia, LAL/Endotoxin.
Cooling Tower Make Up Analysis, Cooling Tower Water Analysis, Cooling Tower Closed Loop Analysis, Boiler Make Up Analysis, Boiler Make Up Water Analysis - Softened, Boiler Feed Water Analysis, Condensate Water Analysis, Closed Loop Water Analysis - Hot, Closed Loop Water Analysis - Cold, AAMI/ Hemodialysis Water Profile.
Alkalinity "M", Alkalinity "OH", Alkalinity "P", Aluminum, Ammonia as NH3-N, Bicarbonate, Barium, Chloride, Conductivity, Conductivity - Neutralized, Copper - Dissolved, Glycols, Fluoride, Hardness - Calcium, Hardness - Magnesium, Hardness -Total, Iron - Dissolved, Manganese, Molybdenum - Molybdate, Monochloramine, Nitrate, Nitrite (as NaNO2), pH, Phosphate - Ortho, Phosphate - Total Inorganic, Phosphonate, Phosphorus - Total, Potassium, Silica - Reactive, Silicon - (as Silica), Silver, Sodium, Stronium, Sulfate, Tolyltriazole, Total Dissolved Solids, Total Organic Carbon, Turbidity, Zinc.
Coliforms - Total (SDWA), Copper (SDWA), Inorganic Chemicals (IOC's) (SDWA), Lead (SDWA), Nitrate (SDWA), Nitrite (SDWA), Synthetic Organic Chemicals (SOC's) with Glyphosate, Synthetic Organic Chemicals (SOC's) without Glyphosate, TTHM/HAA5 (Disinfection By-Products) (SDWA), Volatile Organic Chemicals (VOC's).
Phigenics empowers our clients to prevent disease and injury by improving the safety, defensibility, and value of water management programs. We do not sell water treatment or hazard control chemicals, and we are not driven to provide a solution that is restricted to an internal product line. We are a vested partner in your water management program and provide INDEPENDENT verification and validation services.
Corporate Office / Lab:
Phigenics, LLC
Arkansas Office / Lab:
Phigenics, LLC
Phone: 1.844.850.4087
Client Service Desk: 1.630.733.8029
Fax: 630-717-8528
Email: info@phigenics.com
© 2024 Phigenics, LLC. All rights reserved.
Phigenics is an innovative water management company that meets the increasingly complex needs of facility owners and managers to improve overall water safety while reducing operational (water, chemical, energy) and capital costs. Our innovative solutions and business model represent a clear shift in thinking. We do not sell chemicals or water treatment equipment and we are not driven to provide a solution that is restricted to an internal product line.
Phigenics LLC
3S701 West Ave, Suite 100
Warrenville, IL 60555
Phone: 844.850.4087
Fax: 630-717-8528
Email: info@phigenics.com
© 2022 Phigenics. All rights reserved.